The effect of pistachio Oil  on Important Body Organs in Female Wistar Rats: an Experimental Study with Histopathological Evaluations

Fatemeh Ayoobi (PhD)¹, Maryam Mohamadi (PhD)², Fatemeh Asadi (MSc)³, Nahid Askari (PhD)⁴, Zahra Taghipour (PhD)⁵, Lida Soltani (PhD)⁶, Alireza Nazari (MD)^{7, 8*}

¹ Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

² Occupational Safety and Health Research Center, NICICO, World Safety Organization and Rafsanjan

University of Medical Sciences, Rafsanjan, Iran

³ Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

⁴ Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

⁵ Department of Anatomy, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

⁶ Department of Medical Surgical Nursing, School of Nursing and Midwifery, Geriatric Care Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

⁷ Social Determinants of Health Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

⁸ Department of Surgery, School of Medicine, Rafsanjan University of Medical Science, Rafsanjan, Iran

Received: 03.05.2023  Accepted: 23.06.2023

Abstract

Introduction: Pistachios are a rich source of antioxidants and anti-inflammatory agents, which are widely used in the treatment of diseases affecting the kidneys, liver, heart, and respiratory system. This study aimed to investigate the effects of pistachio oil as a dietary supplement on important bodily organs.

Methods: Twenty-one female rats were used in a randomized design, which included three groups (n=21). Group 1: rats received a normal diet; Groups 2 and 3: rats received a normal diet supplemented with 10 mg/kg and 50 mg/kg of pistachio oil via gavage for 21 days, respectively. Blood samples were collected for biochemical assessments, including blood urea nitrogen (BUN), creatinine (Cr), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), glucose (Glu), triglycerides (Tg), high-density lipoprotein cholesterol (HDL), and total cholesterol (Chol). The liver, kidneys, ovaries, uterus, and brain were harvested for histological evaluations. A significance level of P<0.05 was considered statistically significant.

Results: Our results indicated that the administration of pistachio oil at both doses (10 and 50 mg/kg) significantly increased biochemical parameters, including Glu, Tg, HDL, AST, and ALT (P<0.001). Additionally, both doses of pistachio oil significantly decreased Chol compared to the control group (P<0.001). Chronic consumption of pistachio oil did not induce pathological lesions in any of the examined organs.

Conclusion: Our results demonstrated that the consumption of pistachio oil is suitable because it is effective and safe without any organ toxicity.

►Please cite this article as follows:

Ayoobi F, Mohamadi M, Asadi F, Askari N, Taghipour Z, Soltani L, Nazari A. The effect of pistachio oil  on important body organs in female Wistar rats: an experimental study with histopathological evaluations. Pistachio and Health Journal. 2023;6(1-2):54-60.