Document Type : Original Article
Subjects
Anti-Cancer Characteristics of the pistachio pericarp extract in Combination with carfilzomib in SK-BR3 and MCF10A cell lines
Zahra Mirzaei (MD)1,2, Mehdi Mahmoodi (PhD)1,3, Sadegh Zarei (PhD)1,2, Maryam Mohamadsadeghi (PhD)3, Mojgan Noroozi Karimabad (PhD)1*
1 Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
2 Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
3 Department of Clinical Biochemistry, Afzalipoor Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Received: 02.05.2023 Accepted: 21.07.2023
Abstract
Background: Pistacia vera is a potential source of bioactive components, particularly polyphenols known for their strong antioxidant properties. This study investigated whether pistachio pericarp extract could enhance the efficacy of carfilzomib (CFZ) while reducing its ototoxicity. We assessed the effects of carfilzomib and pistachio pericarp extract on cell viability in the breast cancer cell lines SK-BR3 and MCF10A.
Methods: SK-BR3 and MCF10A cells were cultured in RPMI1640 medium with pistachio pericarp extract (156.25 to 10,000 μg/mL) and CFZ (0.032 to 50 μg/mL) for 24 and 48 hours. The MTT assay evaluated the inhibitory effects of pistachio pericarp extract and CFZ, individually and in combination, on the proliferation of SK-BR3 and MCF10A cells.
Results: The ED50 values of pistachio pericarp extract were 0.228 mg/mL and 0.04 mg/mL for SK-BR3 cells and 0.642 mg/mL and 0.387 mg/mL for MCF10A cells at 24 and 48 hours, respectively. Carfilzomib showed ED50 values of 0.011 µg/mL and 0.00008 µg/mL for SK-BR3 cells, and 0.896 µg/mL and 0.273 µg/mL for MCF10A cells at the same time points.
Conclusion: The results demonstrated a synergistic interaction between pistachio pericarp extract and CFZ in both cell lines at both time points, indicating that the combination therapy was more effective than either treatment alone and may serve as potential molecular targets for various cancers. Future research is recommended to validate the effects in both in vitro and in vivo.
Mirzaei Z, Mahmoodi M, Zarei S, Mohamadsadeghi M, Noroozi Karimabad M. Anti-Cancer Characteristics of the pistachio pericarp extract in Combination with carfilzomib in SK-BR3 and MCF10A cell lines. Pistachio and Health Journal. 2023;6(3-4):22-30.
